NF-κB在成人原发性肾病综合征发病中的作用及机制探讨

Mechanism of NF-κB in Adults with Primary Nephritic Syndrome

目的探讨核因子-κB(nuclear factor kappa B,NF-κB)在成人原发性肾病综合征(pri mary nephritic syn-drome,PNS)发病机制中的意义,研究PNS患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中NF-κB活性对白细胞介素8(interleukin-8,IL-8)水平的调控作用。方法以28例确诊PNS的成人患者(PNS组)和25例健康成人(对照组)为研究对象,抽血、提取PBMC并培养,用酶联免疫吸附法分别检测两组的NF-κB活性吸光度(opticaldensity,OD)值及酶联免疫吸附双抗体夹心法检测IL-8的浓度。再分别用NF-κB的特异性激动剂IL-1β和糜蛋白酶样丝氨酸蛋白酶抑制剂N-甲苯磺基-L-苯乙胺酰氯甲基酮(N-tosyl-L-phenylanyl chloromethyl ketone,TPCK)进行干预后,分别检测两组的NF-κB活性吸光度和IL-8的浓度。结果与正常对照组相比,PNS组NF-κB活性及IL-8浓度均显著增高,差异有统计学意义(P<0.05),且随着NF-κB活性的增强和抑制,IL-8的浓度也相应升高和降低,两者有直线相关关系(P<0.05),而正常对照组无此相关关系。结论NF-κB在成人原发性肾病综合征发病中有重要意义,可能是通过调节IL-8的浓度而发挥其致病作用。

Objective To investigate the effect of nuclear factor kappa B（NF-κB）in pathogenesis of primary nephritic syndrome（PNS）in adults,to study the regulatory effect of NF-κB activity in peripheral blood mononuclear cell（PBMC）on interleukin-8（IL-8）.Methods Twenty-eight patients with PNS and 25 normal adults were in study.Isolated PBMCs were separated from fresh blood in asepsis condition,cultured by RPMI 1640 and divided into 3 groups.Group control was cultured by pure PRMI solution,group IL-1β was cultured by adding and concentration of IL-8 were measured by enzyme-linked immunosorbent assay （ELISA）. Results The activity of PBMC NF-κB and concentration of IL-8 of PNS patients were significantly higher than the normal adults （P〈0. 05）. The activity of PBMC NF-κB of PNS group had linear correlation with the level of IL-8 （P〈0. 05）. Conclusion NF-κB plays an important role in the pathology of PNS in adults by modulating the IL-8 concentrations.