佛手散对先兆流产模型大鼠TNF-α影响的实验研究

Experimental Research of Foshousan of the Impact on Tumor Necrosis Factor-α in Threatened Abortion Model Rats

目的从肿瘤坏死因子-α(TNF-α)水平变化角度探讨活血药对先兆流产模型大鼠的影响及机理。方法大鼠雌雄配对后筛选40只孕鼠,随机分4小组:先兆流产模型对照组(Ⅰ组)及低、中、高剂量给药组(Ⅱ、Ⅲ、Ⅳ组),于妊娠第18～19天处死大鼠,数双侧子宫着床点数以及活胎数。采用放免法测定血清、子宫、胎盘TNF-α浓度。结果中药佛手散对先兆流产模型大鼠的妊娠结局无不良影响。当发生先兆流产时血清及子宫胎盘组织中TNF-α升高,佛手散可降低血清及子宫胎盘组织中TNF-α,改善流产不良结局。结论中药佛手散可改善先兆流产模型大鼠的妊娠结局,不同剂量间无明显差异。

Objective To explore the impact and mechanism of the drug for activating blood circulation on tumor necrosis factor -α （ TNF - α） in threatened abortion model rats. Methods After pairing of rats,40 pregnant rats were selected and randomized into 4 groups, named model control group, low, middle and high dose groups of medication. The rats were sacrificed on the 18th or 19th days of pregnancy. The numhers of bilateral implantation in uterus and living fetus were counted. Radioimmunoassay was adopted to detect TNF -α concentrations of serum, uterus and placenta. In situ hybridization was used to detect the level of IL - 6 mRNA of rats in every group. Results Chinese medicine, Foshousan had no any adverse impact on the pregnant outcome in threatened abortion rats. While threatened abortion occurred,TNF - α ncentration increased in serum and the tissues of uterus and placenta. Fushousan reduced TNF -α concentration in serum and the tissues of uterus and placenta and released the adverse outcomes of abortion. Conclusion Chinese medicine,Foshousan improves the pregnant outcomes of threatened abortion model rats and does not present apparent difference in efficacy among different doses.