HPLC法测定清火栀麦口服液中脱水穿心莲内酯和栀子苷含量

Determination of Dehydroandrographolide and Geniposide in Qinghuozhimai Oral Liquid by HPLC

目的建立高效液相色谱法测定清火栀麦口服液中脱水穿心莲内酯和栀子苷含量的方法。方法采用依利特C18(4.6mm×200mm,5μm)色谱柱,脱水穿心莲内酯的流动相为甲醇-水(55∶45),检测波长为250nm;栀子苷的流动相为乙腈-水(13∶87),检测波长为238nm。结果脱水穿心莲内酯在0.24～1.20μg范围内与峰面积呈良好的线性关系,r=0.9999,平均回收率=97.69%,RSD=1.63%;栀子苷在1.68～6.72μg范围内与峰面积积分值呈良好的线性关系,r=0.9999,平均回收率=101.09%,RSD为2.66%。结论HPLC法能准确、快速地测定清火栀麦口服液中脱水穿心莲内酯和栀子苷的含量。

Objective To establish a method for determining the contents of dehydroandrographolide and geniposide in Qinghuozhimai Oral Liquid by HPLC. Methods Elite C18 column（4.6 mm × 200 mm,5 μm） was adopted. The mobile phase of debydroandrographolide was methyl alcohol -water（ 55：45 ）and the test wave length was 250 nm. The mobile phase of geniposide was methyl cyanides - Water（ 13 ： 87 ） and the wave length was 238 nm. Results Dehydroandrographolide showed a good linear relationship at a range from 0.24 to 1.20 μg with peak area,r = 0. 999 9,the average recover,：, was 97.69% and RSD was 1.63%. Geniposide showed a good linear relationship at a range from 1.68 to 6.72 μg with peak area, r = 0. 999 9, the average recovery was 101.09% and RSD was 2.66%. Conclusion HPLC can determine the contents of dehydroandrographolide and geniposide in Qinghuozhimai Oral Liquid accurately and quickly.