慢性间歇性缺氧对大鼠下丘脑-垂体-性腺轴的影响

The effect of chronic intermittent hypoxia on hypothalamic-pituitary-testicular axis in rats

目的制作可应用于常压慢性间歇性缺氧研究的动物模型,探讨慢性间歇性缺氧对大鼠下丘脑-垂体-性腺轴影响。方法大鼠分别给予空气(对照组,UC组)、间断低氧4周(慢性间歇性缺氧组,CIH组)及复氧4周(复氧组,RH组)处理后用放射免疫法测定血清睾酮(T)、促卵泡生成素(FSH)、促黄体生成素(LH)水平,免疫组织化学测定下丘脑促性腺激素释放激素(GnRH)含量。结果与UC组、RH组比较,CIH组的T、FSH、LH、GnRH明显减低,UC组和RH组无明显改变。结论慢性间歇性缺氧致使大鼠下丘脑-垂体-性腺轴异常,反馈调节紊乱,复氧能使其恢复正常。

Objective：To set up animal model of chronic intermittent hypoxia in normal pressure and study the effect of chronic intermittent hypoxia on hypothalamic - pituitary - testieular axis. Methods： Rats were respectively exposed to room air 4 weeks （ unhandled control , UC ） , intermittent hypoxia 4 weeks （chronic intermittent hypoxia, CIH） , intermittent hypoxia 4 weeks and then room air 4 weeks（restore hypoxia, RH）. Testosterone （T）, follicle - stimulating hormone （ FSH ）, luteotropie hormone （ LH ） in serum of rats were measured by radioimmunity, and gonadotropin - releasing hormone in hypothalamus of rats were measured by immunohistochemistry. Results： The level of T, FSH, LH, GnRH of the rats in the CIH group decreased significantly than those in the UC group and RH group, but there was no differenee between UC group and RH group. Conclusions： Chronic intermittent hypoxia can lead rats＇ hypothalamic - pituitary. - testicular axis to abnormality and disorder of feedback regulation, restore hypoxia can make it normal.