基于基因组改组技术选育多重抗性乙醇酵母

Screening of Multiple-stress Resistant Saccharomyces Cerevisae by Genome Shuffling Methology

本文通过基因组改组(Genome shuffling)技术将酵母菌株的多重耐受性能集中于同一菌株,从而选育出多重抗性的乙醇酵母。以分别经过热灭活和紫外灭活的酵母GZ-0533、GZ-241、GZ-582为亲本菌株,经过3轮基因改组后,筛选得到的28株酵母菌。第二次改组得到的MSR14和第三次改组得到的MSR23对多重胁迫条件的耐受性提高最多,乙醇产量分别达到8.58%和8.86%,较多重抗性最好的出发菌GZ-05相对提高了7%和10.5%。26SrDNA序列分析表明,MSR14和MSR23的26SrDNA序列与酿酒酵母(Saccharomyces cerevisiae)的同源性为100%,均认定为酿酒酵母。

Using genome shuffling technology, the multiple stress resistant yeast was screened in this paper. 28 mutants were obtained by restructuring the genomes of three parent strains. It was found that MSR1 4 obtained from the second shuffling and MSR23 obtained from the third shuffling had the ability of multiple stress tolerance. With the two strains, the ethanol production reached 8.58%（v/v） and 8.86%（v/v） respectively under multiple stress,, which were higher by 7% and 10.5% respective than those of the best parent strain GZ-05. According to the 26SrDNA sequence analysis, both MSR14 and MSR23 were identified as Saccharomyces cerevisiae.