超声波辅助提取海金沙黄酮及其抑菌效果研究

Ultrasound-assisted Extraction of Flavonoids from Lygodium and Its Bacteriostasis Properties

为了进一步研究和开发蕨类植物海金沙资源,以海金沙地上部分为原料,确定海金沙总黄酮的最佳提取剂,并通过单因素试验和三元二次回归设计实验,用SPSS软件对数据进行分析处理,得出海金沙黄酮最佳提取条件。结果表明采用NaOH-乙醇作为提取剂,超声波辅助提取海金沙黄酮的最佳工艺条件是:NaOH-乙醇做提取剂(NaOH浓度0.22%,乙醇浓度60%),料液比1∶30(g/mL),室温浸提2h,超声波功率338W处理130s;此条件下,海金沙黄酮的提取率为8.4%。同时对海金沙总黄酮提取液的抑菌效果进行了分析,结果表明,海金沙黄酮提取液对不同微生物的抑菌效果不同,对细菌(金黄色葡萄球菌、大肠杆菌)有良好的抑制作用,对霉菌(黑曲霉、黄曲霉)无抑制作用。

The flavonoids from Lygodium was extracted with ultrasound-assisted method and the best technological conditions were determined by single-factor and orthogonal experiments. The results showed that the best extraction agent, ratio of liquid to materials, temperature, extraction time, ultrasonic power and treatment time were mixture of 0.22% NaOH and 60% ethanol, 1：30（g/mL）, room-temperature（28℃）, 3 h, 338 W, and 130 s, respectively. Investigation of the antimicrobial activity of the extract showed that extract could effectively inhibit the growth of the bacteria, such as Staphylococcus aureus and E. coli., but have little effect on fungus.