高效液相色谱法测定大米蛋白中的三聚氰胺

Determination of Melamine in Rice Protein with HPLC

建立高效液相色谱仪检测大米蛋白中三聚氰胺含量的方法。样品经固相萃取，采用XDB-C8色谱柱，在240m检测波长进行HPLC分析。该方法简单、快速、重现性好。在0．5～50μg／mL范围内呈良好线性（r^2=0．9999），在添加浓度为2～10mg／kg范围内，三聚氰胺回收率在90％-08％。

A HPLC method was developed for the determination of melamine content in rice protein. For the quantitative analysis, samples were pretreated by solid-phase extraction （SPE） and then analyzed on a XDB-C8 stainless column at 240nm.The method was accurate, rapid and convenient. The liner range of melamine content was 0.5-50μg/mL （r^2=0.9999）. And the recoveries of melamine were 90-98% when the concentration of melamine was of 2-10 mg/kg.