砷急性中毒病人尿中无机砷及其价态测定

Determination on Inorganic Arsenic and Its Valence State in Urine of Patients with Acute Arsenic Poisoning

目的应用氢化物发生—原子荧光分析技术测定砷中毒病人尿液中总无机砷、三价砷(As3+)和五价(As5+)的浓度。方法用6mol/LHCl提取中毒病人尿液中无机砷,在2mol/LHCl条件下测定总无机砷,再利用在强酸条件下,三氯甲烷可以定量将无机砷中As3+萃取到有机相中从而达到分离As3+和As5+,最后分别测定不同价态的砷。结果样品回收率无机砷含量为95.9%～103.5%,As3+为95.6%～101.6%,As5+为98.6%～103.1%;相对标准偏差无机砷为4.26%,As3+为6.68%,As5+为5.38%。结论该方法用于砷化合物急性中毒病人尿中无机砷的测定及价态分析,分析速度快,灵敏度高,干扰少。

[ Objective ] Hydride Generation-Atomic Fluorescence Spectrometry was applied to determine the concentrations of inorganic arsenic, trivalent arsenic （As^3＋ ）and pentavalent arsenic （As^5＋ ）. [ Methods] 6 mol/L HCl was used to extract the inorganic arsenic from poisoned patientg urine. The total concentration was determined under 2 mol/L HCl condition. The method of quantitative extraction of As^3＋ from inorganic arsenic solution into organic phase in strongly acidic conditions was adopted to extract As^3＋and As^5＋ . Finally, the concentrations were measured. [ Results ] Sample recovery rates of inorganic arsenic,As^3＋ and As^5＋ were 95.9% - 103.5%, 95.6% - 101.6%, 98.6% - 103.1% respectively. The relative standard deviation of inorganic arsenic, As^3＋ and As^5＋ were 4.26%, 6.68% and 5.38% respectively. [ Conclusion] The method is suitable for determination of inorganic arsenic and its valence state in urine of patients with acute arsenic poisoning for its rapid analysis, high sensitivity, and less interference.