干预AE和NKCC离子通道对有核红细胞富集效果的观察

The Study of the Nuclear Red Blood Cells' Rich by Interfering Membrane Ionic Channels Selectively

目的:探讨联合干预阴离子通道(AE)和Na+/K+/2Cl-离子共转运体(NKCC)细胞膜离子通道对富集脐血中有核红细胞(NRBC)的效果。方法:采用AE钙离子抑制剂维拉帕米(verapamil)和NKCC通道活性特异性抑制剂呋噻米(ferosemide)联合干预细胞膜,用流式细胞技术观察脐血中胎儿NRBC的富集效果。结果:流式细胞仪检测NRBC的平均检出率干预前为2.4%,干预后为9.7%(u=3.19,P<0.05),联合干预能提高NRBC的富集率4倍。结论:维拉帕米和呋噻米的干预能有效提升NRBC的富集率。

Objective： To investigate the effectiveness of the Nuclear Red Blood Cells ＇ rich by effecting membrane ionic channels-Anion Exchanger and Na^＋/K^＋/2Cl^- Co-transporter in umbilical cord blood. Methods： Make the optimal interfering condition from RBCs on the umbilical cord blood,the rate of cells＇ enrichment by discontinuous density centrifugation on different densities were determined by Flow Cytometry （FCM）. Results： The average purity of NRBCs enrichment in the umbilical cord blood could take from 2.4% to 9.7% （u=3.19, P〈0.05） by interfering, which means to increase about 4 times of enrichment of NRBC. Conclusions： Both Verapamil and Furosemide could promote the rate of NRBC＇enrichment about 4 times.