超干贮藏对菊苣种子活力与抗氧化性的影响

Effects of ultradrying storage on vigor and antioxidase activity of Cichoriun intybus seeds

研究超干处理对菊苣种子活力和抗氧化性的影响,采用硅胶干燥法超干处理菊苣种子,分别得到8.45%,5.17%,3.64%,2.33%和1.69%含水量的种子,密封于双层铝箔袋内放在50℃恒温箱内进行人工老化处理10 d,回水后测量种子活力及抗氧化酶等指标,结果显示老化前超干种子与未超干种子各项指标无明显差异,老化后超干处理的菊苣种子的发芽率、活力指数、脱氢酶、过氧化物酶和过氧化氢酶均高于对照,电导率、丙二醛含量低于对照,在超干种子中含水量为2.33%时贮藏效果优于其他含水量的种子。表明适度含水量的超干处理可以使菊苣种子保持较高的活力,提高了其耐贮藏性,为探讨菊苣种子的适宜贮藏方法提供依据。

In this experiment the effects of ultra drying treatment on vigour and antioxidase activity of C. intybus seeds was studied. Seeds of C. intybus were ultra dried with silica gel to reduce the moisture content to concentrations of 8.45G; 5.17%, 3.64%, 2.33%, and 1.69G. Seeds were then sealed in aluminium foil bags and stored in a calorstat at 50℃ for 10 d. The seed vigor and antioxidase activities were assessed after they were re-moistened. There were no significant changes of seed physiological parameters under ultradrying or non-ul tradrying treatments before temperature treatment. Seed germination rate and vigor index of C. intybus seeds were higher than those of the control under conditions of ultra drying after temperature treatment while the electric conduct peroxidase and ivity and MDA content were lower than those of the control. The activity of dehydrogenase, hydrogen peroxidase were higher than those of the control. For ultra drying of seeds, 2. 33G moisture content was better than other moisture contents for storage effectiveness. The results demonstrated that with an appropriate ultradrying treatment, C. intybus seeds can maintain robust vitality with enhanced storage effectiveness and thus provide a basis for suitable storage of C. intybus Seeds.