麝香保心丸对自发性高血压大鼠心脏保护作用的研究

Protective Effect of Heart-protecting Musk Pill on Heart in Spontaneously Hypertensive Rats

目的探讨麝香保心丸(HMP)对自发性高血压大鼠(SHR)心脏保护作用及其可能的机制。方法将6周龄SHR分为HMP治疗组、高血压模型组,将WKY大鼠作为正常血压组,每组分3批在3个时间点(给药6周、给药14周、停药9周)处死大鼠,尾套法测定血压;FRAP法进行心脏组织总抗氧化能力测定;采用RT-PCR考察炎症相关因子ICAM-1、VCAM-1、IL-1β和TNFαmRNA表达;Western Blot与ELISA法分别考察炎症相关因子ICAM-1、VCAM-1及IL-1β、TNFα蛋白表达。结果HMP对SHR无明显降压作用,但能提高SHR心脏组织总抗氧化能力。PCR、Western Blot与ELISA显示给予HMP 6周和14周后,均能显著抑制SHR心脏炎性相关因子ICAM-1、VCAM-1 m RNA表达及ICAM-1蛋白表达;给药14周后,能显著抑制炎性相关因子IL-1β、TNFαmRNA表达及蛋白表达;停药9周后,仍能抑制炎性相关因子ICAM-1、TNFαmRNA表达及ICAM-1蛋白表达。结论HMP具有独立降压以外的对SHR心脏保护作用,其机制可能与抑制心脏组织炎症反应及降低组织氧化应激有关。

Objective To investigate the protective effect of Heart-protecting musk pill（HMP） on heart in spontaneously hypertensive rats（SHR）,and its possible mechanisms.Methods 6 wk-old male SHR were randomly dicided into the HMP treatment group and model.Age-matched male Wistar-Kyoto（WKY） normotensive rats were used as the control group.Rats in each group were periodically decapitated in batch at three time points（6 week-treatment,14 week-treatment,and 9 week post-treatment）.Systolic blood pressure（SBP） was measured by tail-cuff method.Total antioxidant capacity of heart was evaluated with FRAP method.RT-PCR was used to measure the mRNA expression of inflammation-related mediators（ICAM-1,VCAM-1,IL-1β and TNFα）.The protein expression of inflammation-related mediators（ICAM-1,VCAM-1,IL-1β,TNFα） was determined by Western Blot and ELISA.Results HMP had no marked effect on SBP,but could elevate total antioxidant capacity of heart in SHR.PCR,Western Blot and ELISA showed that HMP could inhibit ICAM-1,VCAM-1 mRNA expression and ICAM-1 protein expression of heart in SHR after 6 weeks and 14 weeks of treatment,inhibit IL-1β,TNFα mRNA expression and protein expression after 14 weeks of treatment,also inhibit ICAM-1,TNFα mRNA expression and ICAM-1 protein expression after 9 weeks of post-treatment.Conclusion HMP has protective effect on heart independent of its hemodynamic effect on SBP.The mechanism may be associated with the suppression of inflammatory response and the decrease of oxidative stress of heart.