摘要
利用正交试验方法优化得到绿豆皮中总黄酮的最佳提取工艺。以芦丁为标准品,采用分光光度法在510 nm下对提取液中总黄酮含量进行测定。通过提取时间、乙醇体积分数、提取温度、固液比与提取次数5个因素的单因素试验,设计L16(45)正交试验筛选最佳工艺。结果表明:提取时间150 m in,乙醇体积分数50%,提取温度80℃,固液比1∶10,提取次数2次,绿豆皮中总黄酮的提取量为3.879 mg/g,平均回收率为100.84%,精密度试验RSD为0.18%。
On the basis of single factor experiment,five influencing factors,namely extracting time,ethanol concentration,extraction temperature,material-liquid ratio and extraction times for extracting flavonoids from mung bean coat were established.Thus a L16（45） orthogonal experiment was applied to optimize the extraction conditions,using spectrophotometry to determine the total flavonoids at 510nm wavelength.Results： The obtained optimal extraction conditions are extracting time 150 min,ethanol concentration 50%, extraction temperature 80℃, material - liquid ratio 1：10 and extraction times 2 times. Under the conditions the extraction rate of flavonoids is 3. 879 mg/g, the average recovery is 100. 84% and RSD is 0. 18%.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2009年第10期124-127,共4页
Journal of the Chinese Cereals and Oils Association
基金
北京市农业技术试验示范项目(20080807)
中国农业科学院人才基金项目(2060302-14)
关键词
绿豆皮
总黄酮
正交试验
分光光度法
mung bean hull
total flavonoids
orthogonal experiment
spectrophotometry
