Smad4基因在大肠癌中的表达及意义

Expression of Smad4 Gene in Colorectal Cancer and Its Significance

目的:探讨Smad4基因在大肠癌中的表达及其与肿瘤分化程度或远处转移的关系。方法:利用免疫组织化学S-P法检测52例大肠癌组织、19例相应的癌旁正常组织中Smad4的表达。结果:Smad4在大肠癌原发灶中的阳性表达率为63.5%(33/52),与癌旁正常组织中的阳性表达率89.5%(17/19)相比,Smad4阳性表达水平显著降低,差别有显著性(P<0.05);Smad4与肿瘤细胞分化程度无关(P>0.05),但与淋巴结转移显著相关(P<0.05)。结论:Smad4的低表达可能是大肠癌发生过程的晚期事件,并可能通过直接或间接的作用促进大肠癌的淋巴结转移。

Objective：To study the expression of Smad4 gene in colorectal carcinoma as well as its association with degree of differentationg and metastasis. Methods：The expression of Smad4 was detected in 52 cases with primary colorectal carcinoma and 19 adjacent normal colorecta tissues by using immunohistochemistrical S -P method. Results：Positive expression rate of Smad4 in primary colorecta cancer tissues was 63.5% （33/52）, compared with the positive rate 89.5% （17/19） in adjacent normal colorec- ta tissues, the Smad4 expression level apparently degraded and there was significant differentiation （ P 〈 0.05 ）. The positive ex- pression of Smad4 had no correlation with degree of differentationg（ P 〉 0.05 ） ,but it was closely associated with lymph node metastasis （ P 〈 0.05）. Conclusion： The low expression of Smad4 could be an advanced stage during the course of the colorectal carcinoma formation. Simultaneously, it also promoted lymph node metastasis directly or indirectly.