人造矿物纤维对肺泡巨噬细胞的毒作用研究

Studies on Cytotoxicity of Man Made Mineral Fiber to Alveolar Macrophage

目的比较１０种人造矿物纤维对肺泡巨噬细胞毒作用的差异。方法选用日本纤维状物质研究协会提供的１０种标准人造矿物纤维（ＪＦＭ纤维），在体外用豚鼠肺泡巨噬细胞（ＡＭ）作为靶细胞，分别对ＪＦＭ纤维作用于ＡＭ后超氧阴离子自由基（Ｏ－２）、过氧化氢（Ｈ２Ｏ２）的产生，谷胱甘肽（ＧＳＨ）及细胞内游离Ｃａ２＋浓度的变化进行了测定。结果１０种ＪＦＭ纤维中，大多数都可引起Ｏ－２和Ｈ２Ｏ２的产生增多、ＧＳＨ含量下降及细胞内游离Ｃａ２＋浓度升高，但其作用均低于温石棉。结论ＪＦＭ纤维的一般细胞毒性均低于温石棉。这些人造矿物纤维虽然作为石棉的代用品使用，但对机体仍会产生一定的有害作用。

Objective To compare the difference in cytotoxicity to human alveolar macrophage caused by ten kinds of man made mineral fiber. Methods Ten kinds of man made mineral fiber prepared by Japan Fibrous Materials Research Association (JFM) were used with alveolar macrophage extracted from guinea pigs in vitro as the target cells. Changes in the production of O - 2 and H 2O 2, levels of glutathione and cellular free calcium ion (Ca 2+ ) in alveolar macrophage were determined. Results Most of the 10 kinds of JFM fiber could cause increased production of O - 2 and H 2O 2, reduction of levels of glutathione and increase of cellular free calcium ion (Ca 2+ ) in alveolar macrophage, to a extent less than that caused by chrysotile. Conclusion In general, the cytotoxicity of JFM fiber was less than that of chrysotile. Although these mineral fiber could be used as substitutes for asbestos, they still could cause certain harm to human bodies.