利用Internet模建IL-6Rα的三维结构

The three dimensional structure modeling of IL 6Rα by Internet

目的：模建人ＩＬ－６Ｒα的三维结构，为构建可拮抗ＩＬ－６作用的ＩＬ－６Ｒα的突变体提供理论依据。方法：以生长激素受体为模板蛋白，在Ｉｎｔｅｒｎｅｔ互联网络上利用Ｓｗｉｓ－Ｍｏｄｅｌ蛋白同源结构模建工具进行ＩＬ－６Ｒα配基结合区的三维结构模建。结果与结论：得到了人ＩＬ－６Ｒα配基结合区的三维结构，并对模型进行了评价，确定了ＩＬ－６Ｒα上与异六聚体复合物一致的作用界面，为构建ＩＬ－６Ｒα的突变体提供了理论依据，并提示双突变体Ｃ２７７Ｄ／Ｈ２８０Ｉ可能既与ＩＬ－６具有高亲和力。

Objective: To model a three dimensional structure of IL 6Rα for directing theoretically the construction of IL 6Rα mutants that can act as antagonist of IL 6. Methods: The growth hormone receptor (GHR) was taken as the template protein to model the IL 6Rα structure. Modeling was carried out through a knowledge based protein modeling tool Swiss Model in Internet. Results and Conclusion: A three dimensional structure model of the ligand binding region of IL 6Rα was obtained, and the possible interaction interface in accord with the heterohexameric IL 6 receptor complex was defined on the model. It provided the direction to the construction of IL 6Rα mutants, and suggested that the double mutant C277D/H280I be an effective antagonist of IL 6 with a higher affinity to IL 6.