Tel／aml1阳性儿童急性淋巴细胞白血病细胞双向凝胶电泳分析

Analysis of Cells in Tel/aml-1 Positive Childhood Acute Lymphoblastic Leukemia by Two-dimensional Gel Electrophoresis

本研究采用双向聚丙烯酰胺凝胶电泳技术建立不同预后tel/aml1阳性儿童ALL白血病细胞的蛋白质表达谱,探讨此临床亚型儿童ALL的发生发展机制。根据初诊时白细胞数、早期治疗反应及临床预后将tel/aml1(+)儿童ALL分为3组:早期复发组、高白细胞组和标危组。取初诊患者骨髓提取蛋白,通过双向聚丙烯酰胺凝胶电泳技术进行细胞蛋白质凝胶电泳,对组间差异蛋白进行图像分析。结果显示:不同组间将tel/aml1(+)儿童蛋白表达谱存在显著差异(大于2倍或小于0.5倍),具有统计学意义(p<0.05)。早期复发病例与标危病例及初诊时高白细胞(>100×109/L)病例相比,有71个蛋白点表达消失,93个新蛋白点出现,37个蛋白点表达上调,23个蛋白点表达下调;标危病例与初诊时高白细胞病例比较,有6个蛋白点表达消失,56个新蛋白点出现,7个蛋白点表达上调,19个蛋白点表达下调。结论:早期复发组病例蛋白表达谱与其他组别存在显著性差异,部分蛋白在儿童ALL的发生发展过程中具有重要作用,可能成为个体化治疗的新的分子指标和药物靶点。

This study was aimed to establish the protein expression profile of tel/aml-1 ＋ childhood acute lymphoblastic leukemia （ALL） with different prognoses by two-dimensional polypropylamide gel electrophoresis and explore the nosogenesis of tel/aml-1 ＋ ALL childhood. On the basis of leukocyte count at new diagnosis, early reaction to therapy and clinical prognosis, the patients with tel/am1-1 ＋ ALL were divided into 3 groups： early relapse group, high leukocyte count group and standard risk group. The bone marrow was taken from newly diagnosed patients for isolating protein, then the protein in leukem/a cells was isolated by two-dimensional polypropylamide gel electrophoresis, the image analysis of differential protein among 3 groups was carried out by using the PDQuest 7.3.0 image analysis software. The results showed that there were significant difference of protein expression profile among 3 groups. As compared with high leukocyte count and standard risk groups, 71 protein spots disappeared, 93 new protein spots appeared, the expression of 37 protein spots was up-regulated and the expression of 23 protein spots was down-regulated in early relapse group. As compared with high leukocyte count group, 6 protein spots disappeared, 56 new protein spots appeared, the expression of 7 protein spots was up-regulated, and expression of 19 protein spots was down-regulated in standard risk group. It is concluded that the protein expression profile in early relapse group is significantly different from other groups. Some proteins may play an important role in pathogenesis of childhood tel/am1-1＋ ALL, and probably become new molecular indicators and targets for individualized treatment.