人早期胎盘SP受体的免疫组织化学研究

Immunohistochemical study of substance P receptor in early human placenta

目的：探讨人胎盘绒毛是否存在P物质（SP）受体及其细胞定位，为其对胎盘及胚胎发育的作用提供形态学依据．方法；采用免疫组织化学ABC法．结果：绒毛两层滋养层细胞，绒毛中轴的基质细胞，毛细血管的内皮细胞，毛细血管腔内的淋巴细胞以及血岛细胞均呈SP受体免疫反应阳性，阳性物质分布于胞质和胞膜上，胞核呈阴性反应．结论：胎盘产生的SP可能在其受体介导下参与胎盘激素合成和释放、胎盘毛细血管发生、微循环以及胚胎免疫功能的调节．

Aim: To study whether substance P receptor(SPR) can exist in human placenta and their cellularlocalization in placental villi, and to supply morphologicalevidence for studying functional significance of the SP inplacenta and fetal development. Methods: Immunohistochemical ABC method was used to the experiment.Results: Both syncytiotrophoblast and cytotrophoblast,stromal cells, capillary endothelium, lymphocytes in capillarycavity,and all cells of blood islet in early human placentaswere all shown SPR immunoreactivity in cytoplasm withnegative nuclei. Conclusion: SP produced by placental villimay take part in regulation of synthesizing and releasing ofplacental hormone, and regulation of capillary developmentand microcirculation in placental villi, and regulation of fetalimmune fuction.