建立增生性瘢痕动物实验模型

Establishment of experimental animal model for hypertrophic scar

目的：建立增生性瘢痕动物模型．方法：选用大耳白兔23只的46个耳朵，产生如下伤口：兔耳腹面作6mm直径的圆形全层皮肤缺损创面100个；1．5cm×1．5cm方形创面12个；圆形全皮加会软骨创面12个；耳背圆形全皮缺损创面24个．结果：耳腹面创面可出现类似人增生性瘢痕样的真皮过度增生，其发生率为80％，增生块最长持续时间已超过伤口愈合后100d．两耳背创面增生块出现率则不及50％，增生块小．组织切片显示：真皮增生厚度为正常的3倍，镜下为大量成纤维细胞增殖，与人增生性瘢痕结构近似，增生块对IFN－γ，TGF-β1的反应与人增生性瘢痕的反应相同．结论：兔耳尤其是兔耳腹面可以产生类似人增生性瘢痕样病理改变，有可能成为研究瘢痕的动物模型．

Aim:To establish animal model for hypertrophicscar. Methods: To total 46 ears of 23 rabbits, and severaltypes of wounds were created as follows: 100 round wounds(d=6mm) and 12 square wounds (15 ×15 mm) with totalskin loses on the ventral side, 24 round wounds (d＝6 mm)on the back side, 12 round wounds (d＝ 6 mm) withcartillage and skin loses on the ventral side. Results:Excessivedermal scarring appears on the ventral side and asts morethan 100 days. Tipical injection of IFN-γ and TGF-β1, showedresponse similar to hypertroophic scar in human body. 50%excessive dermal scarring appears on the back side.Conclusion: Excessive dermal scarring appears after wound close onthe ventral side of rabbit's ear. It might become an animalmodel for the study of hypertrophic scar.