胆囊结石需氧菌和厌氧菌分子生物学研究及意义

A study on molecular biology of aerobic and anaerobic bacteria in disease with gallstone patients

目的：研究胆囊结石细菌脱氧核糖核酸（DNA），提供细菌在胆囊结石核心形成作用中分子生物学证据．方法：采用聚合酶链反应（PCR）对70例胆结石中5种需氧菌和4种厌氧菌DNA进行检测．结果:总的检测出细菌DNA60例（85．71％），其中需氧菌DNA存在43例（61．43％），厌氧菌DNA存在17例（24．29％）．两类细菌DNA共同存在11例（15．71％）．首次从15例结石中检测出幽门螺杆菌DNA．结论：从分子生物学水平证实在胆囊结石中存在细菌．细菌可能是形成结石核心的重要因素．

Aim: To study deoxyribonucleic acid (DNA) ofaerobic and anaerobic bacteria in gallstones and provideevidence of molecular biology by bacteria value in gallstonesformation nuclear. Methods: DNAs of five aerobic and fouranaerobic bacteria were tested with polymerase chainreaction (PCR) in 70 gallstones patients. Results: Measured allDNAs of bacteria were 60 patients (85. 71%), which DNAsof aerobic and anaerobic bacteria were 43 patients (61.43%) and 17 patients (24. 29%), respectively. DoubleClasses coexistent bacteria DNAs were 11 patients (15.71%). DNAs of Helicobacter Pylori were tested in 15gallstones patients at first. Conclusion: Existent bacteria ofgallstones were demonstrated in molecular biology level.Bacteria may be important factor to form gallstone nuclear.