摘要
目的:研究转染p(21WAF1/CIP1)对肾癌的促凋亡作用.方法:采用脂质体介导的逆转录病毒载体将p21(WAF1/CIP1)转入无P21蛋白表达的肾癌细胞系GRC-1中,通过电镜、流氏细胞仪、DNA梯电泳检测细胞凋亡.结果:电镜检测到凋亡细胞,DNA电泳呈梯状.所检测细胞中15.3%出现凋亡.结论:p21(WAF1/CIP1)过表达有促进GRC-1细胞凋亡作用.
Aim: To study the role of p21WAF1/CIP1 genetransfection on renal carcinoma cells apoptosis. Methods:transelectronmicroscope. flowcytometry and DNA elctophoresiswere used to observe cells apoptosis which induced bylipofection-mediated p21WAF1/CIP1 transfection in GRC- lcells.Results: Apoptosis cells could be seen by electron microscope,the 'DNA ladder' appeared on DNA electrophoresis, and anapoptotic peak appeared in front of G1 phase,the apoptoticcells accounted for 15. 3%. Conclusion: It confirmed thatthe overexpression of p21WAF1/CIP1 can induce apoptosis inGRC-1 cells.
出处
《第四军医大学学报》
1998年第6期687-689,共3页
Journal of the Fourth Military Medical University