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番木瓜eIF(iso)4E基因克隆及其结构和表达分析 被引量：3

eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzing in Papaya
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摘要 eIF4E(eukaryotic translation initiation factor4E)家族基因在多种病毒侵染植物过程中起重要作用。应用RT-PCR和RACE方法在番木瓜中克隆了cDNA全长为995bp的eIF4E异构体基因,命名为CP-eIF(iso)4E(GenBank登录号为FJ595992)。其编码的蛋白含有206个氨基酸,蛋白一级结构中的活性部位位点高度保守,三维结构中可能的抗性位点位于帽结合区域(cap-binding pocket)表面。半定量RT-PCR结果表明,CP-eIF(iso)4E在番木瓜叶片中表达较弱,在花瓣中表达最强。 eIF4E （eukaryotic translation ruination tactor 4E） famliy genes pray important roles against virus from infecting plants. A complete cDNA of 995 bp from papaya was cloned using the method of RT-PCR and RACE. It was a isoform eIF4E, so it was named CP-elF（iso）4E （GenBank accession No. FJ595992）. The predicted CP-eIF （iso） 4E protein had 206 amino acids, in which the active site was high conservative in primary structure, and the predicted virus-resistance site was located on the surface of cap-binding pocket in 3-D structure. Semi-quantitative RT-PCR analysis indicated that CP-eIF （iso） 4E expression was lower in leaf and higher in petal of papaya.
作者 言普 沈文涛 高新征 李亚丽 周鹏
机构地区 中国热带农业科学院热带生物技术研究所
出处 《园艺学报》 CAS CSCD 北大核心 2009年第10期1437-1442,共6页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(30760134) 中央级科研院所基本科研业务费项目(ITBBZD0732) 海南省自然科学基金项目(808186)
关键词 番木瓜 eIF(iso)4E 结构分析 基因表达 papaya eIF（iso） 4E structure analysis gene expression
分类号 S667.9 [农业科学—果树学] Q78 [生物学—分子生物学]
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参考文献16

  • 1Gao Z, Johansen E, Eyers S, Thomas C L, Noel Ellis T H, Maule A J. 2004. The potyvirus recessive resistance gene, sbml, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. Plant J, 40:376 -385.
  • 2Kang B C, Yeam I, Li H, Perez K W, Jahn M M. 2007. Ectopic expression of a recessive resistance gene generates dominant Potyvirus resistance in plants. Plant Bioteehnol J, 5 : 526 - 536.
  • 3Leonard S, Chisholm J, Laliberte J F, Sanfacon H. 2002. Interaction in vitro between the proteinase of Tomato ringspot virus (genus nepovirus) and the eukaryotic translation initiation factor iso4E from Arabidopsis thaliana. J Gen Virol, 83 (8) : 2085 -2089.
  • 4Leonard S, Plante D, Wittmann S, Daigneault N, Fortin M G, Laliberte J F. 2000. Complex formation between potyvirus VPg and translation eukaryotic initiation factor 4E correlates with virus infectivity. J Virol, 74 : 7730 - 7737.
  • 5Miyoshi H, Suehiro N, Tomoo K, Muto S, Takahashi T, Tsukamoto T, Ohmori T, Natsuaki T. 2006. Binding analyses for the interaction between plant virus genome-linked protein (VPg) and plant translational initiation factors. Biochimie, 88:329 -340.
  • 6Nicaise V, German-Retana S, Sanjuan R, Dubrana M P, Mazier M, Maisonneuve B, Candresse T, Caranta C, LeGall O. 2003. The eukaryotic translation initiation factor 4E controls lettuce susceptibility to the potyvirus Lettuce mosaic virus. Plant Physiol, 132:1272 -1282.
  • 7Nieto C, Morales M, Orjeda G, Clepet C, Monfort A, Sturbois B, Puigdomenech P, Pitrat M, Caboche M, Dogimont C, Garcia-Mas J, Aranda M A, Bendahmane A. 2006. An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon. Plant J, 48 : 452 - 462.
  • 8Plante D , Viel C, Leonard S, Tampo H, Laliberte J F, Fortin M G. 2004. Turnip mosaic virus VPg does not disrupt the translation initiation complex but interferes with cap binding. Physiological and Molecular Plant Pathology, 64:219 -226.
  • 9Roudet-Tavert G, Michon T, Walter J. 2007. Central domain of a potyvirus VPg is involved in the interaction with the host translation initiation factor eIF4E and the viral protein HcPro. J Gen Virol, 88:1029 - 1033.
  • 10Ruffel S, Dussauh M H, Palloix A, Moury B, Bendahmane A, Robaglia C, Caranta C. 2002. A natural recessive resistance gene against Potato virus Y in pepper corresponds to the eukaryotic initiation factor 4E (eIF4E). Plant J, 32:1067 -1075.

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园艺学报
2009年 第10期

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