摘要
对江西紫金牛属12种植物17个样本及外类群柳叶密花树的核ITS序列和叶绿体trnL-F序列进行最大简约法(MP)和邻接法(NJ)分析,两种方法得到的系统发育树基本一致。基于trnL-F序列所建立的系统发育树将19个样本聚类成6个分支,而基于ITS序列建立的系统发育树将18个样本聚类成2个分支,说明紫金牛属并不是一个单系类群。基于ITS序列建立的系统发育树将腋序组(Ⅰ)和锯齿组(Ⅲ)聚在一起,说明这两个组有较近的关系,而圆齿组(Ⅱ)稍远。基于ITS序列所建立的系统树将4个紫金牛样本聚类成2支,说明紫金牛的群体中至少存在两个核基因组类型,根据染色体数、ITS序列和trnL-F序列综合分析,推测紫金牛很可能是异源四倍体。
The nuclear encoded internal transcribed spacer （ITS） region and the plastid encoded trnL-F region were sequenced for 17 samples from 12 species of the genus Ardisia Sw. and outgroup Rapanea lin- earis. Based on maximum parsimony and neighbor-joining analyses, we reconstructed the phylogenetic relationships of 19 samples of Ardisia. The two methods generated similar trees. Nineteen samples of Ardisia are separated into six clades in trnL-F trees. Eighteen samples of Ardisia are separated into two clades in ITS trees. The results showed that the genus Ardisia Sw. could not be a monophyletic group. The Sect. Akosmos （ Ⅰ ） and Sect. Bladhia （ m ） form a clade in ITS trees, which indicated that they were closely related. Four samples of A. japonica form two clades in ITS trees, which showed that there were at least two types of nuclear genome in A. japonica population. According to analysis of the chromosomes number, ITS sequences and trnL-F sequences, A. japonica is supposed to be a allotetraploid.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第10期1531-1537,共7页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30860027)
