多重聚合酶链反应技术快速鉴定鲍曼不动杆菌

Rapid Identification of Acinetobacter baumannii by Multiplex PCR

目的建立快速鉴定鲍曼不动杆菌菌株的方法。方法本研究建立多重PCR实验技术快速鉴定170株醋酸钙-鲍曼不动杆菌复合体以及对照组的其他菌属14株。结果138株菌的PCR产物扩增出2条条带,为鲍曼不动杆菌,另外32株只扩增出1条条带,为醋酸钙-鲍曼不动杆菌复合体的其他基因型,对照组的菌株没有扩增出条带。结论多重PCR技术的建立为快速鉴定鲍曼不动杆菌提供了一个快速而简便的方式。

Objective To establish a method for the rapid identification of Acinetobacter baumannii. Methods 170 clinical isolates of A. calcoaceticus-A, baumannii complex were detected by rapid multiplex PCR and 14 strains of other species were used as negative control. Results The result shows that there are 138 clinical isolates of Acinetobacter baumannii with amplified two gene fragments and 32 clinical isolates of the other species of A. calcoaceticus-A.baumannii complex with only amplified one gene fragment in the multiplex PCR test. No amplifications were obtained with bacteria belonging to other genera. Conclusion Multiplex PCR technique provides a fast identification technique for Acinetobacter baumannii and Acinetobacter spp.