2型糖尿病患者血浆中COX-2与VEGF的变化及其与尿蛋白水平的相关性研究

The Relationship between Plasma COX-2/VEGF and Albuminuria in Type 2 Diabetic Patients

目的通过检测糖尿病患者血浆中环氧化酶同工酶-2(COX-2)与血管内皮生长因子(VEGF)的变化,了解COX-2及VEGF水平的变化及其与患者尿蛋白定量之间的关系。方法收入51例2型糖尿病患者及47例年龄/性别匹配的健康志愿者,以ELISA法检测其血浆中COX-2与VEGF的水平,同时对研究对象的尿蛋白水平等肾功能指标进行检测,并对两组指标进行相关性分析。结果糖尿病患者血浆COX-2水平(55.58±8.84)ng/mL及VEGF水平(377.59±38.60)pg/mL均显著高于对照组[COX-2(0.89±0.27)ng/mL,VEGF(43.51±8.61)pg/mL](P均<0.01);COX-2与VEGF之间存在正相关,差异有统计学意义(r=0.983,P<0.01),COX-2及VEGF与患者尿蛋白之间均存在正相关,差异有统计学意义(COX-2与尿蛋白r=0.728,P<0.01;VEGF与尿蛋白r=0.769,P<0.01);COX-2及VEGF与患者血肌酐之间均存在正相关,差异有统计学意义(COX-2与血肌酐r=0.649,P<0.01;VEGF与血肌酐r=0.619,P<0.01)。结论2型糖尿病患者血浆中存在明显升高的COX-2及VEGF,并与患者尿蛋白水平及血肌酐水平存在相关性,提示COX-2及VEGF可能参与糖尿病肾病的发病过程。

Objective This study aims to understand the correlation between the changes of cyclo-oxygenase-2 （COX-2）, vascular endothelial growth factor （VEGF） and urinary albumin in type 2 diabetic patients. Methods 51 type 2 diabetic patients and 47 normal control subjects with similar age and gender were enrolled into the study. The plasma level of COX-2 and VEGF was detected by ELISA. The levels of urinary albumin and renal functions were also examined. Results The level of plasma COX-2 （55.58±8.84） ng/mL and VEGF （377.59±38.60） pg/mL in type 2 diabetic patients was significantly higher than the control subjects [ COX-2 （0.89±0.27） ng/mL, VEGF （43.51±8.61） pg/mL, Both P〈0.01 ]. In diabetic patients, a positive correlation between the level of COX-2 and VEGF was observed （r=0.983,P〈0.01）. Both COX-2 and VEGF showed positive correlation with the level of urinary proteins （COX-2 and albumin, r=0.728,P〈0.01; VEGF and albumin, r=0.769,P〈0.01）. The levels of COX-2 and VEGF were also positively correlated with the patients＇ serum creatine level （COX-2 and serum creatine r=0.649,P〈0.01; VEGF and serum creatine r=0.619,P〈0.01 ）. Conclusion The levels of plasma COX-2 and VEGF are elevated in type 2 diabetic patients and the levels are positively correlated with urinary albumin and serum creatine. Both COX-2 and VEGF may be involved in the pathogenesis of diabetic nephropathy.