白细胞介素-8样免疫阳性物质在大鼠垂体前叶中的存在和分布

Existance and localization of interleukin-8 like immunoreactive substances in rat anterior pituitary

目的：检测白细胞介素-8（IL－8）样免疫阳性物质在大鼠垂体前叶中的存在和分布．方法：用抗入IL－8多克隆抗体行ABC免疫组织化学染色．结果：正常大鼠垂体前叶存在IL－8样免疫阳性物质；阳性细胞主要位于大鼠垂体前叶的浅层区域；大鼠出生3d内已有免疫阳性物质存在，在15d左右形成其分布规律；IL－8样免疫阳性物质主要存在于GH细胞．结论：正常大鼠垂体前叶稳定性存在IL－8样免疫阳性物质，提示IL－8可能影响垂体前叶细胞增殖和（或）激素分泌，从而在神经内分泌免疫网络中发挥一定的作用。

Aim: To investigate the existence andlocalization of IL-8 like immunoreactive substances in rat anteriorpituitary(AP). Methods: ABC immunohistochemistry withanti-human IL-8 polyclonal antibody was used for staining.Results: Many IL-8 like immunoreactive substances werefound in rat AP and mainly localized in the shallow layer ofAP. A weaker immunostaining appeared in AP of postnatalwithin the birth of 3d of rats. The special localizationpattern were formed about 15d old rats. IL-8 likeimmunoreactive substances are mostly localized in GH cells.Conclusion: IL-8 like immunoreactive substances constantlylocalized in normal rat AP cells, suggesting that IL-8 mayinfluence the AP cell proliferation and/or hormone secretion,and play some roles in the cross-talk between the immuneand neuroendocrine system.