TRPC6对肝癌细胞增殖的介导

Cell proliferation in hepatocellular carcinoma by TRPC6

目的瞬时受体势（TRP）是一种新发现的钙通道，是当前细胞信号转导领域内的研究热点之一。以往有关TRP通道的研究主要集中在神经系统，现已开始向其它研究领域扩展。笔者推测，TRP通道可能与肝癌细胞的增殖有关。该研究将证实此推测。方法应用TRPC通道抑制剂（SKF-96365）和RNAi技术抑制该通道蛋白在肝癌细胞中的表达和功能，观察TRPC被抑制前后肝癌细胞体外增殖能力的改变。结果研究发现，应用SKF-96365和siRNA方法阻滞TRPC6的通道功能后，肝癌细胞（HepG2和SMMC-7721）的增殖被明显抑制。结论该研究证明TRPC6通道介导了肝癌细胞的增殖，为肝癌细胞增殖机制的研究提供了新的思路，探索了肝癌临床治疗新的细胞膜上的靶点，同时也拓展了TRP通道的研究领域。

Objective Transient recepter potential （TRP） is a kind of novel Ca^2＋-permeant channel and one of the hot topics in the current basic researches. The researches on TRP were mostly focused on nervous system in the past, and now they come to extend to other areas. We hypothesized that TRP channels should he likely related to cell proliferation of hepatocellular carcinoma（HCC）. This study was designed to confirm this hypothesis. Methods After inhibiting the function of TRPC channels by pharmacological means （SKF 96365） and RNAi, we observed the effect of this inhibition on cell proliferation in vitro. Results After inhibition of TRP channels by SKF-96365 and RNAi, the proliferation of two HCC cell lines of HepG2 and SMMC-7721 was significantly repressed. Conclusion The TRPC6 channels are implicated in HCC cell proliferation. This study not only provides new insights into proliferation of HCC cells and subsequent cancer intervention strategies but also opens a new area for TRP channel study.