颅内生殖细胞肿瘤的免疫组织化学研究

Immunohistochemical study of intracranial germ cell tumors

目的：探讨各种颅内生殖细胞肿瘤中免疫组织化学染色的诊断意义。方法：对７６例颅内生殖细胞肿瘤进行５种抗体的免疫组织化学染色。结果：胎盘碱性磷酸酶（ＰＬＡＰ）阳性率８２％（６２／７６），绒毛膜促性腺激素（ＨＣＧ）阳性率３０％（２３／７６），胎盘催乳素（ＨＰＬ）仅占５％（４／７６），甲胎蛋白（ＡＦＰ）和妊娠特异性β１糖蛋白（ＳＰ１）在卵黄囊癌中阳性率１００％（５／５）。结论：ＰＬＡＰ可用于生殖细胞肿瘤与其他肿瘤鉴别诊断。ＡＦＰ和ＳＰ１可检出畸胎瘤中不易察觉的卵黄囊癌成分。ＳＰ１阳性可能提示肿瘤恶性程度高，预后差。

Objective: To evaluate the value of immunohistochemical stain in diagnosis for various intracranial germ cell tumors. Methods: 76 cases with intracranical germ cell tumors were studies by immunohistochemical method with five different monoclonal antibodies. Results: The positive rate of placenta alkaline phosphatase (PLAP), human chorionic gonadotropin (HCG) and human placental lactogen (HPL) was 82% (62/76), 30% (23/76) and 5% (4/76) respectively. In yolk sac tumors, the positive rate of alfafetoprotein (AFP) and pregnancy specific β 1 glycoprotein (SP 1) was 100% (5/5). Conclusion: PLAP can be applied for differentiating diagnosis of germinomas from other tumors. The indistinguishable yolk sac tumors cell in teratomas can be found by AFP staining combined with SP 1 staining. The latter indicate a higher malignant degree.