同步荧光法同时测定苯二酚中邻苯二酚和对苯二酚

Simultaneous determination of catechol and hydroquinone by synchronous fluorescence spectrometry

建立固定波长同步荧光法同时测定邻苯二酚和对苯二酚的分析方法。邻苯二酚和对苯二酚在纯水介质中,固定波长差(△λ=λem-λex)为19nm时进行荧光光度同步扫描时,两组分得到互不干扰的同步荧光峰,邻苯二酚和对苯二酚的最大同步荧光峰分别为298nm和315nm,借此分别测定混合液中两组分的含量。邻苯二酚和对苯二酚在1.0×10-6～9.0×10-5mol/L和1.0×10-6～1.0×10-4mol/L浓度范围内与同步荧光强度呈线性关系,相关系数分别为0.9986和0.9991,检出限分别为8.2×10-7mol/L和5.4×10-7mol/L。该法用于测定水样中邻苯二酚和对苯二酚,回收率分别为83%～107%和92%～100%。

A synchronous fluorescence method for simultaneous determination of catechol and hydroquinone has been established. In pure aquo-medium, by fixing the wavelength interval （△A） as 19 nm, the sychronous fluorescence scanning for eatechol and hydroquinone is performed fo get the maximum peaks at 298 nm and 315 nm, respectively. On the basis of the fact, the content of both is determined. The concentrations of cateehol and hydroquinone are linear with the intensity of synchronous fluorescence in the concentration range of 1.0× 10^-6 - 9. 0 × 10^-5 mol/L and 1.0 × 10^-6 -- 1.0 × 10^-4 mol/L, with correlation coefficients of 0. 998 6 and 0. 999 1 and detection limits of 8.2 × 10^-7 mol/L and 5.4× 10^-7 mol/L. The recoveries of catechol and hydroquinone are found in the range of 83%-- 107% and 92%--100%, respectively.