摘要
目的研究17β-雌二醇(17β-E2)对乳腺癌细胞MCF-7增殖作用及其对雌激素受体相关受体(ERRα)的调控作用。方法分别用10-12、10-11、10-10、10-9、10-8、10-7、10-6mol/L的17β-E2作用于MCF-7细胞24h,以DMSO作为溶剂对照,采用MTT法测定细胞增殖情况。分别用10-10、10-9、10-8、10-7mol/L17β-E2作用于MCF-7细胞24h后,采用RT-PCR法检测ERRα表达水平的变化。结果10-12、10-11、10-10、10-9、10-8、10-7mol/L剂量17β-E2作用于MCF-7细胞24h后,对细胞的增殖率分别为112.09%,122.09%,123.42%,120.46%,124.60%,109.23%,均能促进细胞生长,并且随着受试物浓度的增加,细胞增殖效应增加,在10-8mol/L剂量时达到最大,而10-6mol/L剂量的17β-E2作用于MCF-7细胞24h后,其增殖率为67.97%,对细胞生长的产生抑制。10-10、10-9、10-8、10-7mol/L17β-E2作用于MCF-7细胞24h后,ERRα的表达水平上调。结论一定剂量范围内17β-雌二醇对MCF-7细胞有增殖作用,并且可以上调ERRα表达,提示ERRα在调节乳腺癌细胞生长中可能发挥着重要作用。
Objective To understand the effects of 17β-estradiol on MCF-7 cell proliferation and the regulation of estrogen receptor-related reeeptor(ERRct). Methods MCF-7 cells were treated with 17β-E2 at the doses of 10-^12、10^-11、10^-10、10^-9、10^-8、10^-7 and 10^-6 mol/L for 24 h. Dimethyl sulfoxide were used as the solvent control. MTT method was used to determine inhibitory rate of MCF-7 proliferation, and the cells were treated with 17β-E2 at the doses of 10^-10、10^-9、10^-8 and 10^-7 mol/L for 24 h. RT-PCR method was used to detect the expression level of ERRor. Results Cell proliferation were promoted by exposure to 17β-E2 at the doses of 10-^12、10^-11、10^-10、10^-9、10^-8、10^-7 and 10^-6 mol/L,the proliferation rate were 112.09%,122.09%,123.42%,120.46%,124.60% and 109.23% respectively, however, cell growth was inhibited at 10^-6 mol/L of 17β-E2. The expression of ERRct in MCF-7 cells was upregulated at the doses of 10^-10, 10^-9, 10^-8 and 10^-7 mol/L when cells were treated with 17β-E2 for 24 h. Conclusion In certain dosage range,cell proliferation and expression of ERRα can be promoted by 17β-E2,which suggests that ERRor should play an important role in the process of MCF-7 cells growth.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第10期875-877,共3页
Journal of Environment and Health
关键词
雌激素
雌激素受体相关受体α
乳腺癌
增殖作用
Estrogen
Estrogen receptor-related receptor alpha
Breast cancer
Proliferation effect