三种方式染毒丙烯酰胺对雄性小鼠睾丸细胞DNA损伤的影响

DNA Damage in Testicular Cells Induced by Acrylamide through Different Exposure Routes in Mice

目的研究腹腔注射、灌胃、皮肤染毒丙烯酰胺(AA)对小鼠睾丸细胞DNA损伤的影响。方法将24只清洁级健康雄性昆明小鼠随机分为4组,分别为空白对照组、腹腔染毒组、灌胃染毒组和皮肤染毒组,每组6只。染毒剂量为25mg/kg,每天1次,连续染毒5d。首次染毒后第6天称体重和睾丸重,计算睾丸系数。采用单细胞凝胶电泳实验对彗星细胞的尾长、尾部DNA%、尾矩(tail moment,TM)、Olive尾矩(olive tail moment,OTM)进行检测。结果染毒后腹腔染毒组和灌胃染毒组小鼠体重均低于空白对照组,差异有统计学意义(P<0.01)。与空白对照组比较,腹腔染毒组、灌胃染毒组和皮肤染毒组小鼠的睾丸重量及其脏器系数均较低,差异均有统计学意义(P<0.05或P<0.01)。与空白对照组比较,腹腔染毒组、灌胃染毒组和皮肤染毒组小鼠睾丸细胞DNA的尾长、尾部DNA%、尾矩、Olive尾矩均较高,差别有统计学意义(P<0.05或P<0.01)。各染毒组两两比较发现,睾丸细胞DNA的尾长、尾部DNA%、尾矩、Olive尾矩间差异均有统计学意义(P<0.05),均以灌胃染毒组最高,腹腔染毒组次之,皮肤染毒组最低。结论丙烯酰胺可致雄性小鼠睾丸细胞产生DNA损伤,且以灌胃染毒方式产生的毒性作用最为敏感。

Objective To know DNA damage effect in testicular cells in mice induced by acrylamide through three exposure routes, intraperitoneal injection, oral exposure and skin exposure. Methods Twenty-four healthy male KM mice were randomly divided into 4 groups,including the blank control group,intraperitoneal injection group,oral exposure group and skin exposure group,6 in each. The exposure dose was 25 mg/（kg.d）, for 5 consecutive days,once a day. On the sixth day after the first exposure,the changes of body weight and testis coefficient were observed. The tail length,tail DNA% ,tail moment,olive tail moment of testicular cells were detected by single cell gel electrophoresis. Results The body weights of intraperitoneal injection group and oral exposure group decreased significantly （P〈0.01）. Compared with the blank control group,the testis weights and testis coefficients of intraperitoneal injection group, oral exposure group, and skin exposure group were all decreased significantly （P〈0.05 or P〈0.01 ）. The indexes of testicular cells DNA damage in the experimental groups were higher than the blank control group significantly （P〈0.05）, oral exposure group was the highest, followed by intraperitoneal injection group,and skin exposure group was the lowest. Conclusion Acrylamide can induce DNA damage of testieular cells in mice. The toxic effects of oral exposure are most serious.