快速进展性牙周炎患者的白细胞趋化功能

Chemotactic response of polymorphonuclear neutrophil to FMLP and IL 8 in rapidly progressive periodontitis patients

目的了解我国快速进展性牙周炎患者的外周血中性多形核白细胞是否存在趋化功能异常。方法选择细菌源性的趋化肽和宿主源性的趋化因子（白细胞介素８）作为趋化剂，采用微孔滤膜法（改良的ＢｏｙｄｅｎＣｈａｍｂｅｒ法）检测１６例快速进展性牙周炎患者的外周血中性多形核白细胞的趋化功能，并与１４名年龄相当的健康个体进行对照。结果快速进展性牙周炎患者的外周血中性多形核白细胞对细菌源性的趋化肽和白细胞介素８的趋化反应与健康组差异无显著性。结论中性多形核白细胞趋化功能的异常可能并不是快速进展性牙周炎的主要易感因素。

Objective It has been reported that 48%～85% of rapidly progressive periodontitis (RPP) are associated with a defect in polymorphonuclear neutrophil (PMN) chemotactic function. However, some reports failed to detect any defect in PMN chemotaxis in RPP patients. Further, the influence of race on neutrophil function has been suggested. The purpose of the present study was to determine the PMN chemotactic function in Chinese RPP patients. Methods Neutrophils were obtained from heparinized peripheral blood of 16 RPP and 14 periodontally healthy control subjects matched for age and sex. Cells were isolated by Percoll discontinuous density gradient centrifugation. Chemotaxis was evaluated using modified Boyden chamber. (N formyl methionyl leucyl phenylalanine, FMLP) (10 8 mol/L) and IL 8 (100μg/L) which is a more specific neutrophil chemokines were used in this study. The results were determined by staining the filters followed by visual counting under microscopy at 600×. Data was analyzed using the Students' t test and the value of RPP was compared with that of control subjects(>mean±2 s or <mean±2 s ).Results and Conclusion The results showed all RPP patients had normal PMN chemotactic behavior.