TNFα检测在药物过敏性休克死因鉴定中的价值

The Value of TNFα Testing in Evaluation of Death due to Drug Anaphylactic Shock

目的分析某些药物过敏性休克致死者血清中肿瘤坏死因子α(tumor necrosis factor-alpha,TNFα)检测水平的变化,探讨TNFα检测在药物过敏性休克死因鉴定中的应用价值。方法采用双抗体夹心酶联免疫吸附试验法(ELISA)检测尸体心血中TNFα的水平。结果实验组TNFα检测值为(131.6±9.4)pg/mL,感染性疾病对照组TNFα检测值为(87.3±6.4)pg/mL,正常对照组TNFα检测值为(17.2±4.5)pg/mL,各组间差异有统计学意义(P<0.05)。结论血清TNFα检测可以作为高度怀疑药物过敏性休克案例的死因鉴定的指标之一。

Objective To analyze the changes of the serum TNFα level in cadavers of drug anaphylactic shock（DAS） and to explore the significance of the TNFα testing in evaluation of death caused by DAS. Methods The level of serum TNFα from cardiac blood was determined by double antibody-sandwich ELISA method. Results The level of TNFα in the experimental group was （131.6±9.4）pg/mL, while the level in the infectious diseases control group and the normal control group were （87.3±6.4） pg/mL and （17.2±4.5） pg/mL, respectively. There was statistically significant difference among the groups （P〈0.05）. Conclusion The serum TNFα level may be used as one of the indexes for evaluation of death caused by highly suspected drug anaphylactie shock