病毒性心肌炎猝死心肌MCP-1表达及其法医学意义

Expression of Monocyte Chemoattractant Protein-1 in Sudden Death due to Viral Myocarditis and Its Medicolegal Significance

目的探讨单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)在病毒性心肌炎(viral myocarditis,VMC)所致猝死者心肌组织中的表达及其意义。方法运用改良的免疫组织化学方法,观察VMC猝死组和对照组心肌组织中MCP-1的表达情况,应用统计学处理,比较两组间的表达差异。结果20例VMC猝死组中有17例MCP-1染色阳性;而20例非VMC猝死对照组仅4例局部散在MCP-1弱阳性表达,其余均阴性。VMC组MCP-1阳性表达率明显高于对照组(P<0.01)。结论MCP-1的免疫组织化学检测可作为法医病理学诊断VMC所致猝死较为客观的病理形态学指标之一。

Objective To explore the expression and significance of monocyte chemoattractant protein-1 （MCP-1） in myocardium in sudden death caused by viral myocarditis（VMC）. Methods To investigate the expression of MCP-1 in VMC sudden death group and control group using improved immunohistochemical technique and to compare the difference of the expression of MCP-1 between two groups with statistical method. Results In VMC sudden death group, MCP-1 was positively expressed in 17 of 20 cases. While only 4 of the 20 cases in the control group showed a mildly positive expression sparsely in myocardium, and the rest cases were completely negative. The rate of positive expression of MCP-1 in VMC group was obviously higher than that of the control group（P〈0.01）. Conclusion The expression of MCP-1 detected by immunohistochemistry provides an objective morphologic evidence for the diagnosis of VMC sudden death in forensic pathology.