摘要
目的研究蛋白质的二级结构。方法介绍蛋白质二级结构定量分析方法,综述FT-IR在蛋白质二级结构研究中的应用进展情况,并对其应用前景进行了展望。结果FT-IR分析技术是一种高效、快速研究蛋白质结构的现代分析技术。结论为了获得更精确的信息,须将FT-IR技术与生物样品的处理与实验设计相结合。
Aim To study protein secondary structure. Methods By introducing the method of quantitative analysis of protein secondary structure, the application progress of the research on protein secondary structure by Fourier transformation infrared spectroscopy was briefly reviewed. Meanwhile, the prospect for its application was examined. Results Fourier Transform Infrared Spectroscopy technique was a highly efficient and rapid modern analysis technique of protein secondary structure.Conclusion In order to obtain more precise information, FT-IR technology should be combined with the handling of biological samples and experimental design.
出处
《宝鸡文理学院学报(自然科学版)》
CAS
2009年第3期47-53,共7页
Journal of Baoji University of Arts and Sciences(Natural Science Edition)
基金
宝鸡文理学院重点科研项目(ZK0792)
宝鸡文理学院重点科研项目(ZK0830)
关键词
FT-IR
蛋白质二级结构
二阶导数谱
去卷积
曲线拟合
Fourier transformation infrared spectroscopy(FT-IR)
protein secondary structure
secondary derivative spectrum
deconvolution
curve fitting