采用固体复合酶测定果糖二磷酸钠含量的方法研究

Study on determination of sodium fructose diphosphate by solid compound enzyme

目的:建立固体复合酶活力测定方法及采用固体复合酶测定果糖二磷酸钠(FDP)含量的酶反应方法。方法:在固体复合酶作用下,通过测量还原型辅酶Ⅰ(NADH)在340nm处吸收度的变化来测定果糖二磷酸的含量。结果:果糖二磷酸钠在0.1～0.6mg·mL-1范围内线性关系良好(r=0.9997),注射用果糖二磷酸钠平均回收率为99.3%(n=9);果糖二磷酸钠注射液平均回收率为99.0%(n=9)。结论:本法方法简便、准确,专属性强,可用于果糖二磷酸的含量测定。

Objective：To establish a method for the determination of enzymatic activity of solid compound enzyme and established a method of enzymatic reaction for the determination of sodium fructose diphosphate（FDP） by solid compound enzyme. Method：The solid compound enzyme was used in the determination of sodium fructose diphosphate by absorption change of NADH at 340 nm. Result：There was a good linear relationship of sodium fructose diphosphate within the range of 0. 1 - 0.6 mg ·mL^-1 （ r = 0. 9997 ）. The average recovery of sodium fructose diphosphate for injection was 99.3% （ n = 9）. The average recovery of sodium fructose diphosphate injection was 99.0% （ n = 9）. Conclusion ： The method was simple, accurate, specific and suitable for the determination of sodium fructose diphosphate.