高效液相离子排阻色谱法测定血液透析液中醋酸根含量

HPLC determination of acetate in dialysate

目的:建立一种测定血液透析液中醋酸根含量的方法。方法:采用高效液相离子排阻色谱法测定血液透析液中醋酸根的含量。色谱条件:色谱柱为Rezex ROA-Organic AcidH+(150mm×7.8mm,8μm);流动相为0.0025mol·L-1硫酸溶液,流速:0.5mL·min-1;检测波长:220nm。结果:醋酸根线性范围是1.64～6.54mmol.L-1(r=0.9994),平均加样回收率为100.6%,RSD=0.08%(n=5)。结论:此方法操作简便,测定时间短,准确度高,透析液中其他离子对测定影响小。

Objective ： To introduce a determination method of acetate in dialysate. Method： High liquid chromatography （HPLC） was used to determine acetate. A under the chromatographic conditions ： Rezex ROA - Organic Acid H＋ （150 mm×7.8mm,8μm）column;mobile phase was 0. 0025 mol·L^-1 sulfuric acid solution at a flow rate of 0.5% mL ·min ^-1, detection wavelength was 220 nm. Results： The linear correlation of acetate was 1.64 - 6.54 mmol· L^-1 with correlation coefficient of 0. 9994. The average recovery was 100. 6% , RSD = 0.08% （ n = 5 ）. Conclusions：The results showed that the method is accurate, simple and rapid for analysis of acetate in dialysate.