铈(Ⅳ)-抗坏血酸-奎宁化学发光体系的研究和应用

Determination of quinine by Ce(Ⅳ)-ascorbic acid chemiluminescence system

目的:建立一种Ce(Ⅳ)-抗坏血酸-奎宁化学发光体系快速测定奎宁的化学发光分析新方法。方法:在硫酸介质中,抗坏血酸可以显著增敏Ce(SO4)2-奎宁产生的化学发光,从而试验以流动注射化学发光法测定奎宁注射液。结果:在优化的试验条件下,该法测定奎宁的线性范围为1.0×10-6～1.0×10-4g·mL-1,检出限为3.7×10-7g·mL-1,其RSD为1.6%(c=5.0×10-6g·mL-1,n=11)。结论:方法的选择性较高,线性范围较宽,并成功应用于奎宁针剂含量的测定,扩大了具有荧光特性药物的分析范围。

Objective：a new chemiluminesence method combined with flow injection technique was developed for the determination of quinine. Method：aseorbic acid could greatly enhance the CL intensity of quinine and Ce （IV） in vitriol as a medium. , thus, Quinine injection was determined with t chemiluminesenee method combined with flow injection technique. Result：Under the optimized conditions, the linear range for quinine was 1.0×10^-6-1.0×10^-4g·mL^-1 with a detection limit of 3.7×10^-7g·mL^-1and the relative standard deviation was 1.6% （c=5.0×10^-6g·mL^-1,n=11 ）. Conclusion：Quinine injection was successfully determined with the suggested method in enlarged analytical range by fluorescence characteristic due to its more higher selectivity and its wider linear range.