2种用油酸诱导的幼猪急性肺损伤模型血流动力学变化的对比分析

Comparison of the hemodynamic characteristic in two acute lung injury models induced by oleic acid in piglets

目的：对比以“近似二次打击”的方法和传统方法构建的油酸诱导的幼猪急性肺损伤模型的血流动力学差异。方法：外科操作稳定30min后，12只幼猪随机分为传统诱导组（T组）和“近似二次打击”诱导组（H组），T组油酸连续经右心耳注射，H组采取近似于“近似二次打击”的方法，分2阶段间隔1h经右心耳连续注射油酸。于各时间点记录血流动力学指标，并取动脉血行血气分析检查。注射油酸后3h处死实验动物。结果：T组有2只幼猪死于油酸应用后发生的急剧的血压下降；T组血流动力学改变较H组剧烈，尤其以油酸注射后的5—30min最为剧烈。实验过程中2组左心房压无显著变化，油酸注射后肺动脉压力显著升高。结论：以油酸按“近似二次打击”法构建的急性肺损伤模型的血流动力学较传统法稳定，2种方法构建的模型均能达到急性肺损伤模型的标准。

Objective：To compare the hemodynamic characteristics in two acute lung injury animal models in piglets induced by oleie acid. Method：12 Chinese mini-piglets were randomized into two groups： group T （received oleie acid-ethanol solution in traditional manner） and group H （received oleie acid-ethanol solution in two-hit manner）. Hemodynamic and pulmonary function data were measured. Result： Two piglets in group T died of extremely rapid decline of systemic blood pressure. Group T showed more drastic hemodynamic changes than group H especially during the period of 5 to 30 minutes after oleie acid administration. Group T and group H produced comparable severe lung injury. Conclusion： Oleic acid leads to severe pulmonary hypertension which results in hemodynamie fluctuation in oleic acid-induced acute lung injury model. The two-hit acute lung injury animal model has slighter changes in hemodynamies than traditional method-induced model.