摘要
【研究目的】花生(Arachis hypogaea L.)是世界上重要的油料作物。近年来,花生分子生物学发展迅速,但低效的再生和遗传转化系统严重制约着利用基因工程改良花生品种的研究,该研究旨在利用嫁接的方法提高花生再生和转化苗的成活率,缩短再生周期。【研究方法】分别采用劈接、斜接、插接的方法将再生和转化的花生苗嫁接到实生花生砧木上。【研究结果】利用嫁接技术将组培苗或转基因苗嫁接到实生苗砧木上,不同嫁接方法成活率达到70%～100%,远远高于转基因幼苗直接诱导生根移栽的成活率。【主要结论】实验证明,嫁接可提高花生再生和转化苗的成活率,可广泛应用于花生基因工程育种研究中。
Peanut（Arachis hypogaea L.） is one of the most important oil crops in the world,belonging to the Leguminosae family and the Papilionacea subfamily,native to South America.Inefficiency of peanut regeneration system retarded its improvement through gene engineering.In this study we investigated the use of grafting to improve the survival rate of tissue cultured and transgenic plants.Plants of 14 days after germination were used as Rootstock,and different grafting methods and plant management after grafting were investigated. The survival rate of different grafting methods ranged from 70%-100% which is much higher than the survival rate ob- tained by directly transplanting transgenic plants after root induction.
出处
《中国农学通报》
CSCD
北大核心
2009年第20期63-67,共5页
Chinese Agricultural Science Bulletin
基金
国家高技术研究发展计划项目"花生脂肪酸代谢相关基因的分离与功能研究"(2006AA10A114)
山东省农业科学院高技术自主创新基金"花生籽粒全长cDNA库的大规模测序及脂肪酸合成相关键酶基因的分离和功能分析"(2006YCX030)
"花生品质相关功能基因挖掘及高效转基因技术体系研究"(2007YCX001)
关键词
花生
嫁接
组织培养
转基因
成活率
Arachis hypogaea
grafting
tissue culture
gene transformation
survival rate
