定量PCR快速检测动物食品中沙门菌污染

Rapid Detection of Salmonella Bacteria Contaminated Animal Food with Real Time PCR

沙门氏菌是动物性食品中的重要食源性致病菌,建立其快速检测方法对动物性食品的质量控制具有重要作用。根据沙门氏菌16srDNA、dT fermentation等基因或DNA功能区序列设计PCR引物,通过比较3种不同的基因组DNA提取方法,提取待检样品总DNA,进行多基因定量PCR检测。结果表明,该方法简单快速且灵敏度高,在动物食品中的检测灵敏度可达10cfu/g,整个检测时间在10h以内。说明该方法对检测动物中沙门氏菌具有特异性高且灵敏、快速等特点,适用于快速、准确地检测动物中沙门氏菌的需要。

Salmonella is an important food borne pathogenic bacteria in animal food,establish a rapid detection methods plays an important role in animal food quality control.Three protocols were used to extract total DNA from one food sample from animal,and DNA products from the three protocols were all amplified by the primer of 16S rRNA gene and dT fermentation with Real Time PCR.Results show that this method is simple,rapid and high sensitivity,the detection sensitivity in animal food can reach up to 10 cfu/g,suggest such method may be used fast testing the salmonella pollution in animal food.