控制NH_4^+的浓度对解淀粉芽孢杆菌产中温α-淀粉酶的影响

Effects of controlling ammonium in Bacillus amyloliquefaciens on α-amylase fermentations

在解淀粉芽孢杆菌生产中温α-淀粉酶的发酵过程中,利用人造沸石与膨润土吸附菌体代谢过程中产生的NH4+对于提高酶活力是有利的。实验表明,添加1.5%人造沸石(40～60目)、添加2%人造沸石(20～40目)、添加2%膨润土时都能够有效地提高酶活力,其中添加1.5%的人造沸石(40～60目)可提高20%。

The addition of synthetic zeolite and bentonite is very useful to control the NH4^＋ level in fermentation of Bacillus amyloliquefaciens to product α- amylase. The experiment indicated, when the concentration of synthetic zeolite（40-60 mesh） 1.5% ,the synthetic zeolite（20-40 mesh）and bentonite was 2%,it could effectively increase the yield of the enzyme.The addition of 1.5% of synthetic zeolite（40-60 mesh） can increase 20% yield of the enzyme over the uncontrolled experiments.