无花果多糖分离、纯化工艺的研究

Study on purification of total ficus carica polysaccharide by macroporous adsorption resin

目的:筛选纯化无花果多糖的最佳大孔树脂及最佳工艺条件。方法:采用静态吸附分离法筛选大孔树脂,并采用动态吸附分离法确定纯化工艺条件。结果:确定层析条件为:上柱溶液pH=8;洗脱速度2mL/min(柱体积30mm×300mm);NaCl浓度为0.03mg/mL。结论:AB-8大孔树脂对无花果多糖有良好的吸附和分离性能,可以达到最佳工艺条件纯化分离多糖,适合于工业化生产。

Methods：The optimum macroporous resin and process were optimized for the purification of total ficus carica polysaccharide （FCPS）. Methods： The static adsorption and separation were used in investigation of macroporous adsorption resin, the dynamic adsorption and separation were used in studying the condition of adsorption and separation. Results： The operation parameters on absorption and elution were established. The density and optimum technological conditions were followed ： wining up velocity 2ml/min（ column volume 30mm × 300mm） ,pH 8,the density of NaCl 0.03mg/mL.Conclusion.The results showed that the AB-8 was the best for adsorbing and separating, and it serves as a good reference for the purification process of the total FCPS. Therefore, the above technique is suitable to industrial production.