摘要
利用气相色谱(GC)建立了普洱茶中17种有机磷农药残留的测定方法。样品中农药残留2次用乙腈提取;弗罗里硅土层析柱净化中依次用30mL三氯甲烷-乙酸乙酯(9∶1,V/V)、30mL乙酸乙酯-甲醇(1∶1,V/V)作为洗脱剂,毛细管柱DB-17分离,火焰光度检测器FPD测定。结果表明,17种有机磷农药3个添加水平的平均回收率为67.23%~108.6%,相对标准偏差2.22%~9.86%。检出限为0.006~0.015mg/kg。该方法具有测定有机磷农药种类多、净化效果好、准确度和精密度高等优点,符合茶叶农药残留国内外检测标准。
The method for determination of 17 organophosphorus pesticide residues in puer tea was developed.The sample was extracted by acetonitrile with a homogenizer.The 17 oganophosphorus pesticide residues in puer tea were separated from each other through dual capillary columns DB- 17 and determined with dual flame photometric detectors(FPD).Under above conditions,the average recoveries of 17 oganophophorus pesticides at spiked levels of 0.05-0.5mg/kg ranged from 62.23% to 108.6% ,and the relative standard deviations(RSD) were 2.22%-9.86%. The minimum detection limits were 0.006-0.015mg/kg.This method has the merits of good purifying effects, high accuracy and precision. It is accorded with pesticide residues testing standards in tea at home and abroad.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第10期321-323,共3页
Science and Technology of Food Industry
基金
云南省科技计划项目(2006NG16)
关键词
普洱茶
有机磷农药
多残留
气相色谱法
测定
puer tea
organophosphorus pesticide
residues
gas chromatography
determination