银杏叶提取物对大鼠心肌缺血-再灌注损伤的保护作用

Effects of Ginkgo Biloba Extract on Myocardial Ischemia Reperfusion Injury in Rats

目的探讨银杏叶提取物对大鼠心肌缺血-再灌注损伤引起的氧化应激损伤的保护作用。方法将SD大鼠随机分为3组,假手术(Sham)组、缺血-再灌注损伤(MIR)组和银杏叶提取物组,每组10只。建立心肌缺血-再灌注模型。银杏叶提取物在造模前给药14d。再灌注结束后采血,测定丙二醛(MDA)、超氧化物歧化酶(SOD)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、乳酸脱氢酶同工酶1(LDH1),测量心肌梗死面积。结果银杏叶提取物能明显降低血浆中AST、LDH、LDH1和MDA的含量,提高SOD活性,减少心肌梗死面积。结论银杏叶提取物对大鼠心肌缺血-再灌注损伤引起的氧化应激损伤的有保护作用。

Objective To investigate the protective effect of ginkgo biloba extract on oxidative stress injury induced by myocardial ischemia reperfusion in rats. Metlaod Thirty healthy SD rats were randomized into 3 groups： sham group, MIR group, ginkgo biloba extract treatment group. Myocardial IR was carried out by ligation of left anterior descending coronary artery for 30min followed by reperfusion for 60 min. Ginkgo biloba extract was administered 14 days before the ligation. Blood was collected at the end of reperfusion for detecting changes of aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, lactate dehydrogenasel （LDH1）, superoxide dismutase （SOD）, and malondialdehyde （MDA）. The myocardial infarct size was also estimated. Results Ginkgo biloba extract pretreating decreased the contents ofAST, LDH, LDH1 and MDA, and the myocardial infarct size, and increased the activity of SOD. Conclusion Ginkgo biloba extract exerts protective effects on myocardial ischemia reperfusion injury.