四君子汤对衰老大鼠胃黏膜CD4^+T细胞的影响

Effects of Sijunzi Decoction on CD4^+T Cells in the GastricMucosa of the Aging Rat

目的:探讨四君子汤对D-半乳糖致衰大鼠胃黏膜内CD4+T细胞的数量变化,为四君子汤对衰老大鼠胃黏膜免疫功能的恢复提供形态学依据。方法:雄性w istar大鼠30只,随机分对照组、模型组和实验组,实验组和模型组用D-半乳糖颈部皮下注射,实验组同时灌服四君子汤,对照组、模型组给予等量的生理盐水灌胃,持续42天,取胃窦,用免疫组化SP法显示黏膜内CD4+T细胞,在生物显微镜下计数。结果:对照组CD4+T细胞数为128±9.5,模型组CD4+T细胞数为38±6.4,与对照组相比,明显减少,两者比较有显著差异(P<0.001);实验组CD4+T细胞数为121±10.4,与对照组比较差异不明显(P>0.05),与模型组比较有明显差异(P<0.001)。结论:四君子汤可以提高衰老大鼠胃黏膜内CD4+T细胞数,具有延缓胃黏膜免疫衰老作用。

objective ： To explore the quantitative change of Sijunzi decoction to CD4^＋ T Cells. And to provide morphological evidence for recovery of the mucosal Immune function in the gastric mucosa of the aging rat. Method： 30 male Wistar rats were randomly divided into control group, model group and experimental group; model group and experimental group are injected with D - galactose on nape while experimental group took Sijunzi decoction and control group and model group were given the same amount of normal saline lasting for 42 days; With the gastric antrum, take count of the positive ceils in the mucous membrane with immunohistochemistry to display CD4^＋ T cells in the gastric mucosa. Result ： the numbers of CD4^＋T cells in control group were 128 ± 9. 5, in model group were 38 ± 6. 4, comparison with control group , the quantitative of the CD4^＋ T cells in model group decreased significantly in the control group （P 〈0. 001 ） ; the quantitative of CD4^＋T in experimental group were 121 ± 10.4, comparison with control group, the quantitative of CD^＋ T ceils have no apparendy different （ P 〉 0.05 ）, comparison between experimental group and model group, the quantitative of CD4^＋T cells are apparent difference （P 〈0. 001 ）, Conclusion： Sijunzi decoction can increase the quantitative of CD4^＋T cells of the gastric mucosa of the aging rat , and can delay the aging of gastric mucosal immunity.