微量量热法对不同产地板蓝根药材品质的评价

Evaluation of Quality of Isatis indigotica from Different Habitats by Microcalorimetry

目的:评价不同产地板蓝根药材的品质。方法:采用微量量热法,测定不同产地板蓝根作用于大肠杆菌的热谱曲线,以生物热力学参数为指标评价其活性的强弱。结果:不同产地板蓝根的生长热力学参数(k1、k2、P1m、T1m和P2m、Tm2)呈现规律性波动,第一指数生长速率(k1)可客观地评价板蓝根品质。结论:生物热力学方法可作为评价板蓝根药材品质的方法之一。

OBJECTIVE： To evaluate the quality of Isatis indigotica from different habitats. METHODS： The thermal-spectra curves of Escherichia coli treated with I. indigotica from different habitats were determined by microcalorimetry, and the activity of I.indigotica was evaluated with biothermodynamic parameters as index. RESULTS： The biothermodynamic parameters（k1、k2、Pm^1、Tm^1和Pm^2、Tm^2） fluctuated regularly, and the growth rate of first exponential phase（k 1） served as an objective parameter for the quality evaluation of I.indigotica from different habitats. CONCLUSION： Biothermodynamic method serves as one of the methods for the quality evaluation of I.indigotica.