黄瓜花叶病毒卫星RNA T1株系的功能研究

Function of Satellite RNA T1 Strain of Cucumber Mosaic Virus

采用RT-PCR在(cucumber mosaic virus,CMV)侵染番茄(Lycopersicon esculentum)中获得一卫星RNA(T1sat,登陆号DQ785472)。以CMV-Fny作为T1sat辅助病毒,CMV-Fny和CMV-Fny+T1sat分别接种烟草(Nicotiana tabacum)和番茄2种寄主植物。接种3 d后,RT-PCR检测寄主系统叶中卫星RNA发现,T1sat均存在于烟草和番茄中,处理寄主的总RNA电泳分析结果也表明:以CMV-Fny为辅助病毒,T1sat在烟草和番茄中均能高含量积累。其中T1sat减轻CMV-Fny在烟草中症状,而CMV-Fny与T1sat混和接种则引起番茄产生坏死症状。接种7 d和14 d后,采用Realtime RT-PCR分析T1sat对这2种寄主中CMV-Fny各基因组影响的结果显示,T1sat均减少烟草和番茄中病毒基因组的含量,但在番茄中T1sat的抑制效应比在烟草中明显。本研究结果提示:T1sat分别减轻和加重CMV-Fny在烟草和番茄中的病毒症状反应,且T1sat均降低CMV-Fny在烟草和番茄中的基因组含量。

A strain of satellite RNA （Tlsat）was cloned by RT-PCR from cucumber mosaic virus （CMV）infected-tomato （Lycopersicon esculentum）and full length of Tlsat was 337 nt （Accession Number：DQ785472）. Mixture of CMV-Fny virion and transcripts RNA from Tlsat cDNA clone was co-inoculated on the seedlings of Nicotiana tabacum,Lycopersicon esculentum, respectively. At 3 days post-inoculation （dpi）, RT-PCR detection results showed that T lsat all existed in the systemic leaves of N. tabacum and L. esculentum. When relative accumulation of Tlsat in the inoculated leaves of host plants was analyzed by Reahime RT-PCR,we found that Tlsat depressed the level of CMV-Fny 1a,2a,2b,3a and CP on N. tabacum and L. esculentum,but the effect of Tlsat on CMV-Fny genomic RNAs accumulation on N. tabacum was more severe than that on L. esculentum. Tlsat attenuated symptoms induced by CMV-Fny on N. tabacum,and the presence of Tlsat aggravated symptom expression caused by CMV-Fny on L. esculentum.