二黄散清除hrHPV的机制

Mechanism of Huang San Ⅱ on hrHPV

目的:探讨含砷复方中药"二黄散"对人乳头状瘤病毒(hrHPV)的作用机制及其治疗宫颈hrHPV阳性者的效果。方法:将二黄散作用于宫颈hrHPV阳性者的宫颈表面。治疗前和治疗后6月分别采用杂交捕获二代法(HC-Ⅱ)检测宫颈局部HPV转阴率,酶联免疫吸附试验ELISA检测血清干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、白介素4(IL-4)、IL-10水平。结果:治疗后HPV转阴率明显上升、IL-4及IL-10水平均下降,IFN-γ和TNF-α水平上升,治疗前后结果比较均具有显著性差异,且HPV的变化与上述因子水平改变相关。结论:二黄散可能通过调节Th1/Th2的免疫平衡清除hrHPV病毒,可以作为临床治疗hrHPV阳性患者的有效手段。

OBJECTIVE To evaluate the mechanism of the compound Chinese medicine including arsenic ＂Huang San Ⅱ ＂ on hrHPV and its clinical efficacy on positive hrHPV infectors. METHODS Huang San Ⅱ was used to the patients with cervical positive hrHPV infection. HC-Ⅱ technique was employed to detect the rate of negative conversion before treatment and 6 months later after treatment. We also estimated the varying serum levels of IFN-γ, TNF-α, IL-4, IL-10 by EI,ISA. RESULTS In our study, as the rate of negative conversion ascending , IL-4 and IL-10 levels were all significantly decreased after treatment, while IFN-γand TNF-α values increased spurtly. These variations above all had notable significancy. Further analysis showed there had some correlation between HPV and these factors. CONCLUSION Huang San Ⅱ may regulate Th1/Th2 im mune balance to cleanup HPV. It is of benefit to positive HPV infectors.