人参中有机磷农残的基质固相分散-HPLC同时快速分析

Simultaneous and Double-quick Analysis of Organophosphorus Pesticide Residues in Ginseng by Matrix Solid-phase Dispersion and High Pressure Liquid Chromatography

建立了基质固相分散-高效液相色谱法(MSPD-HPLC)对中药材人参中的三种有机磷农药甲胺磷、甲基对硫磷和乙基对硫磷残留量进行快速分析.将HPLC法应用于有机磷农药残留量的检测,分别采用中性Al2O3和体积比为8%的乙酸乙酯-正己烷作为MSPD的分散剂和洗脱剂,对分析方法的质量控制问题进行了详细讨论,并对不同种类的人参中药材进行了准确的定性定量分析.该方法的线性范围为0.20～10.00mg·L-1,相关系数(R)大于0.99815,相对标准偏差(RSD)均小于11.54%,检测限(LOD)低于0.017mg·L-1,样品的加标回收率为87.29%～92.43%.其线性范围、相关系数、准确度、精密度和LOD等指标均满足有机磷农残分析的要求.

A rapid analysis method based on matrix solid-phase dispersion （MSPD） and high pressure liquid chromatography （HPLC） was established to analyze three kinds of organophosphorus pesticide residues in ginseng. The HPLC method was applied to determine organophosphorus pesticide residues. Al2O3 was used as dispersant, and the mixed solution of acetic ester and hexane with an 8% volume proportion was used as eluant. The quality control problem of this analytical method was discussed particularly. The qualitative and quantitative analysis were performed exactly for different varieties of ginsengs. The linear range of this method was 0.20- 10.0 mg·L^-1, and the linear correlation coefficient （R） was higher than 0.99815. The relative standard deviation （RSD） was less than 11.54% with the recovery values ranging from 87.29% to 92.43%. The limit of detection （LOD） was lower than 0.017 mg·L^-1. The method indexes of linear range, accuracy, precision and LOD can well satisfy the request of organophosphorus pesticide residue analysis.