肿瘤坏死因子-α对内皮细胞组织因子和组织因子途径抑制物表达的影响

Effects of TNF-α on the expression of TF and TFPI in vascular endothelial cells

目的:观察肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)对人脐静脉内皮细胞(HUVECs)表达TF及TFPI的影响。方法:设不同时间组,TNF-α10ng/mL分别培养0h、1h、3h、6h、12h、24h,另设不同溶度组,TNF-α0ng/mL、1ng/mL、10ng/mL、20ng/mL、50ng/mL、100ng/mL分别培养6h;ELISA法测定TF及TFPI抗原的表达;RT-PCR检测TF及TFPI基因的表达。结果:各浓度的TNF-α对HUVECs的细胞活力无明显影响;TNF-α促进TF抗原的表达,并呈剂量和时间依赖关系,在TNF-α10ng/mL作用6h时最明显(P<0.01);TNF-α促进TFmRNA的表达,并呈剂量和时间依赖关系,在3h时达到高峰(P<0.01);TNF-α对HUVECs表达TFPI抗原及TFPImRNA无明显影响。结论:TNF-α可以诱导HUVECs细胞表达TF;TNF-α对HUVECs细胞TFPI的表达无明显影响。

Objective To observe the effects of TNF-α on the TF and TFPI antigen expression in HUVECs. Methods HUVECs were cultured to TNF-α （10 ng/mL） for the six various lenghts of time（0 h, 1 h, 3 h, 6 h, 12 h, 24 h）, and the various concentration of TNF-α （0, 1, 10, 20, 50,100ng/mL） for 6h. TF and TFP1 antigen were detetermined by ELISA; TF mRNA and TFPI mRNA were examined by RT-PCR. Results There were no significant difference in cell viability between treated groups and untreated groups; TNF-α increased TF antigen expression in HUVECs in a dose or time-dependent manner, reaching a maximum level with TNF-α 10 ng/ml after 6 hours （P 〈 0.01 ） ; TNF-α increased TF mRNA expression in HUVECs in a dose or time-dependent manner, reaching a maximum level with TNF-α 10 ng/mL after 3 hours （P 〈 0.01 ） ; There was no effect on the TFPI antigen and mRNA exprssion induced by TNF-α in HUVECs. Conclusions TNF-α can induce the TF expression in HUVECs; There has no effect on TFPI expression induced by TNF-α in HUVECs.